Coding
Part:BBa_K4268002:Experience
Designed by: J. Aubrey, J. Alvarenga, L. Buchanan, J. Reyes, D. Yashinski Group: iGEM22_SUNY_Oneonta (2022-07-26)
The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.
The gel indicates that all five colonies are likely to contain the correct insert and thus, the Capsid Protein was successfully cloned into a Level 0 Golden Gate Assembly basic part.
Applications of BBa_K4268002
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UNIQ151c85e9cd7f1e47-partinfo-00000000-QINU UNIQ151c85e9cd7f1e47-partinfo-00000001-QINU